ABSTRACT
@#Toxocara canis is a major parasite that infects many animals with high risk of human infections. This study aims at assessing the immunization with gamma radiationattenuated infective stage on rats challenged with non-irradiated dose. Level of vaccine protection was evaluated in liver and lung regarding parasitological, histopathological, biochemical and molecular parameters. Fifty rats were enrolled in three groups: group A (10 rats) as normal control; group B (20 rats) subdivided into subgroup B1 (infected control) and subgroup B2 infected then challenged after 14 days with the same dose of infection (challenged infected control); and group C (20 rats) subdivided into subgroup C1 vaccinated with a dose of 800 gray (Gy) gamma-radiated infective eggs (vaccine control) and subgroup C2 vaccinated then challenged on 14th day with same number of infective eggs (vaccinated-challenged). Tissues were stained with Haematoxylin and Eosin (H&E) for histopathological studies. Biochemical studies through detection of nitric oxide (NO) and Caspase-3 were conducted. Extent of DNA damage by Comet assay was assessed. Vaccinated-challenged subgroup revealed a marked reduction in larvae in tissues with mild associated histological changes. In addition there was accompanied reduction of NO, Casepase-3 level and DNA damage compared to the control infected group. It could be concluded that vaccination of rats with a dose of 800Gy gamma radiation-attenuated infective stage improves immune response to challenge infection and drastically reduces the morbidity currently seen.
ABSTRACT
@#Toxocara canis is widely distributed parasite that not only presents in definitive hosts but also occurs in paratenic hosts including human. Larvae migrate throughout the somatic tissue causing severe inflammatory and pathological reactions. This study aims to detect the effect of infection with Toxocara canis on testis of rats regarding histopathological, histochemical and immunohistochemical changes and amelioration of these changes with either vaccination with gamma radiation-attenuated embryonated eggs or with herbal treatment with thyme. The study was conducted on eighty rats classified into four groups (20 each): Group A (normal control); Group B (infected control); Group C infected and treated with thyme oil (thyme-treated); and Group D vaccinated with 800 Gy gamma radiation-attenuated embryonated eggs, and challenged with the same number of eggs (vaccinated-challenged). Testicular tissues were stained with Haematoxylin and Eosin (H &E) for histopathological study. Periodic acid Schiff’s (PAS), bromophenol blue (BPB) and Feulgen’s reaction for carbohydrates, proteins and DNA, respectively were done to examine histochemical changes. Immunohistochemical study was done through expression of TGF-β1 and caspase-3. Infected control group B showed severe histopathological changes with marked decrease in PAS +ve materials, total proteins and DNA and enhanced expression of Transforming growth factor- β1 (TGF-β1) and caspase-3. Moderate changes were observed in testicular tissues of group C treated with thyme. Slight changes were detected in vaccinated-challenged group D. It was concluded that Toxocara canis infection causes marked hispathological, histochemical and immunohistochemical changes in testicular tissues of rats that can be ameliorated by vaccination with radiation-attenuated infective stage or treated with thyme; however vaccination is more effective in protection.
ABSTRACT
Dente invaginado é uma anomalia do desenvolvimento caracterizada por invaginação dos tecidos dentários que varia de ligeiro aumento da fosseta do cíngulo a um profundo sulco que pode se estender até o ápice dentário. O objetivo deste trabalho foi discutir um caso clínico de dente invaginado tipo II em incisivo lateral superior em que foi realizado o tratamento endodôntico convencional. Posterior ao acompanhamento de 11 anos e frente aos exames clínicos e imaginológicos observou-se tratamento endodôntico satisfatório e regressão da rarefação óssea. Baseado nisso, foi possível concluir que o tratamento endodôntico convencional é uma alternativa viável para tratamento de dente invaginado tipo II.
Dens invaginatus is a developmental anomaly characterized by invagination of the dental tissues ranging from slight rise of the pit to a depth of the cingulate sulcus and can be extended to the apical portion of the tooth. The aim of this study was to relate a case of dens invaginatus type II in upper lateral incisor in which a conventional treatment was realized. After eleven years-follow up and based on clinical and images exams, it was observed that the endodontic treatment was efficient and the bone rarefaction reduced. Based on this, it was possible to conclude that conventional endodontic treatment is a viable alternative for type II dens invaginatus.
ABSTRACT
Microbial fuel cells are the electrochemical exchangers that convert the microbial reduced power, generated via the metabolism of organic substrate, to electrical energy. The aim of this study is to find out the rate of produced electricity and also treatment rate of simulated wastewater of food industries using dual chamber microbial fuel cell [MFC] without mediator and catalyst. MFC used in this study was consisted of two compartments including anaerobic anode chamber containing simulated food industries wastewater as synthetic substrate and aerobic cathode chamber containing phosphate buffer, respectively. These two chambers were separated by proton exchange membrane made of Nafion. Produced voltage and current intensity were measured using a digital ohm meter and the amount of electricity was calculated by Ohm's law. Effluent from the anode compartment was tested for COD, BOD5, NH3, P, TSS, VSS, SO42- and alkalinity in accordance with the Standard Methods. In this study, maximum current intensity and power production at anode surface in the OLR of 0.79 Kg/m3.d were measured as 1.71 mA and 140 mW/m2, respectively. The maximum voltage of 0.422 V was obtained in the OLR of 0.36 Kg/m3.d. The greatest columbic efficiency of the system was 15% in the OLR of 0.18 Kg/m3.d. Maximum removal efficiency of COD, BOD5, NH3, P, TSS, VSS, SO42- and alkalinity, were obtained 78, 72, 66, 7, 56, 49, 26 and 40%, respectively. The findings showed that the MFC can be used as a new technology to produce electricity from renewable organic materials and for the treatment of different municipal and industrial wastewaters such as food industries
Subject(s)
Bioelectric Energy Sources , Electrochemical Techniques , Food Industry , ElectrodesABSTRACT
Background: Worldwide primary angioplasty is a recommended strategy of reperfusion in patient with acute myocardial infarction as because it ensures reperfusion of the infarct-related vessels more than 90% where as, with thrombolytics it is only 60-70%. Methods: It is a retrospective observational study includes all patients treated with primary angioplasty at United Hospital from Between March 2007 to August 2010. Total 237 consecutive patients with acute myocardial infarction were treated with primary angioplasty were included. Those presented beyond 12 hours of onset of chest pain, in cardiogenic shock, resuscitate and intubated before the procedural were excluded from the study. Results: Majority (76%) of the patient were male, age was minimum 28 years and maximum 80 years, 41.5% were diabetics, 58.4% were hypertensive, 43.5 %were dyslipidaemic, 17% were smoker, 29.3% with positive family history. Fifty seven percent patients presented with anterior MI, 42 % with inferior MI and 1 % with lateral MI. Left anterior descending (LAD) is the most common vessel involved (S7%), followed by Right coronary artery (RCA) 31 %, Left circumflex artery (LCD 8 %, Ramus 1.3 % and Graft vessel 2.7%. Our door to balloon time was minimum 23 min, maximum 184 min. We used drug eluting stents for most of the patients , GP IIbIIIa receptor Mockers used in 50% cases and thrombus suction device were used when indicated. We faced complications like arrhythmias in 24% hypotension in18 %, no flow or slow flow in 45%, cardiac arrest in 3% and coronary perforation in 1. %. Our overall survival was 97.9 %. Conclusion: Primary angioplasty is a emerging area in the context 149-154of our country . Many of the new centers start this novel strategy which helps to save many lives. Primary angioplasty is feasible and safe method of reperfusion in patient with acute myocardial infarction in our center.